unable to access TWAIN data source

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unable to access TWAIN data source

Peter Hawkes
Hi all

I sent this out a couple of months ago, but had no response, so am appealing again for help...our only alternative for speeding up our measurement procedure seems to be spending a fortune on an additional Leica LAS module, for which we simply do not have funds. So - if you have any solutions to the issue below, please let me know!

We are having trouble getting ImageJ to access a live image from a Leica microscope camera - is it possible to do this?  

The setup we have is an i7 PC running Win 7 Pro 64 bit, connected to a Leica microscope / camera  via Firewire B.  Leica LAS software runs the motorfocus and camera and other programmes such as Photoshop are able to access the live camera image via File / Import / Leica DFC camera.  A prerequisite for installing LAS was to install Leica DFC Twain 7.4.0

We have followed the instructions for installing ImageJ including adding Twain_6.class to the plugins folder, adding Morina framework to ImageJ/jre/lib/ext folder.  ImageJ runs fine and we can carry out measurements on a saved image.  However we would like to be able to measure on a live image to improve efficiency (we are working with 3-D specimens that need to be correctly orientated for each measurement and it would be very tedious to have to capture and save an image with magnification info etc for each measurement).

When we try to access the camera via plugins/TWAIN 6 we get an error mesage " SK.gnome.TWAIN.TwainException: No TWAIN Data Source installed "

Can anyone please help - are we doing something wrong, or is it not possible to access a data source directly within ImageJ via Twain?  Does the code perhaps need to be tweaked to call the Leica DFC Twain 7.4.0 rather than Twain 6? Or do we need to install a Twain 6 driver and if so, where can we find this?  Please bear in mind that we are completely innocent of any programming know-how!

Best wishes
Peter





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Re: unable to access TWAIN data source

Krs5
Dear Peter,

You not really write what Leica camera you have but did you try MicroManager (https://micro-manager.org/)? This is a imageJ release focused on image acquisition using automated microscopes and here (https://micro-manager.org/wiki/BaumerOptronic) is info about Leica cameras supported.

Best wishes

Kees


Dr Ir K.R. Straatman
Senior Experimental Officer

Advanced Imaging Facility

Centre for Core Biotechnology Services
University of Leicester
www.le.ac.uk/advanced-imaging-facility<https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.le.ac.uk%2Fadvanced-imaging-facility&data=02%7C01%7Ckrs5%40leicester.ac.uk%7C1b858e336e704219470108d7d1a93493%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C637208396975917696&sdata=4dA2%2FO90BthnwF71o6N1uCTaUaUrpG8g%2F2GdR4u5400%3D&reserved=0>



________________________________
From: Peter Hawkes <[hidden email]>
Sent: 01 April 2020 14:43
To: [hidden email] <[hidden email]>
Subject: unable to access TWAIN data source

Hi all

I sent this out a couple of months ago, but had no response, so am appealing again for help...our only alternative for speeding up our measurement procedure seems to be spending a fortune on an additional Leica LAS module, for which we simply do not have funds. So - if you have any solutions to the issue below, please let me know!

We are having trouble getting ImageJ to access a live image from a Leica microscope camera - is it possible to do this?

The setup we have is an i7 PC running Win 7 Pro 64 bit, connected to a Leica microscope / camera  via Firewire B.  Leica LAS software runs the motorfocus and camera and other programmes such as Photoshop are able to access the live camera image via File / Import / Leica DFC camera.  A prerequisite for installing LAS was to install Leica DFC Twain 7.4.0

We have followed the instructions for installing ImageJ including adding Twain_6.class to the plugins folder, adding Morina framework to ImageJ/jre/lib/ext folder.  ImageJ runs fine and we can carry out measurements on a saved image.  However we would like to be able to measure on a live image to improve efficiency (we are working with 3-D specimens that need to be correctly orientated for each measurement and it would be very tedious to have to capture and save an image with magnification info etc for each measurement).

When we try to access the camera via plugins/TWAIN 6 we get an error mesage " SK.gnome.TWAIN.TwainException: No TWAIN Data Source installed "

Can anyone please help - are we doing something wrong, or is it not possible to access a data source directly within ImageJ via Twain?  Does the code perhaps need to be tweaked to call the Leica DFC Twain 7.4.0 rather than Twain 6? Or do we need to install a Twain 6 driver and if so, where can we find this?  Please bear in mind that we are completely innocent of any programming know-how!

Best wishes
Peter





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Re: unable to access TWAIN data source

Peter Hawkes
Dear Kees

Thank you very much - that has indeed solved our problem and we are now able to take measurements using ImageJ from the live camera image...this will be a great help!

Best wishes
Peter
  ----- Original Message -----
  From: Straatman, Kees (Dr.)
  To: [hidden email] ; Peter Hawkes
  Sent: Wednesday, April 01, 2020 4:09 PM
  Subject: Re: unable to access TWAIN data source


  Dear Peter,


  You not really write what Leica camera you have but did you try MicroManager (https://micro-manager.org/)? This is a imageJ release focused on image acquisition using automated microscopes and here (https://micro-manager.org/wiki/BaumerOptronic) is info about Leica cameras supported.


  Best wishes


  Kees


  Dr Ir K.R. Straatman
  Senior Experimental Officer

  Advanced Imaging Facility

  Centre for Core Biotechnology Services
  University of Leicester
  www.le.ac.uk/advanced-imaging-facility







------------------------------------------------------------------------------

  From: Peter Hawkes <[hidden email]>
  Sent: 01 April 2020 14:43
  To: [hidden email] <[hidden email]>
  Subject: unable to access TWAIN data source

  Hi all

  I sent this out a couple of months ago, but had no response, so am appealing again for help...our only alternative for speeding up our measurement procedure seems to be spending a fortune on an additional Leica LAS module, for which we simply do not have funds. So - if you have any solutions to the issue below, please let me know!

  We are having trouble getting ImageJ to access a live image from a Leica microscope camera - is it possible to do this?  

  The setup we have is an i7 PC running Win 7 Pro 64 bit, connected to a Leica microscope / camera  via Firewire B.  Leica LAS software runs the motorfocus and camera and other programmes such as Photoshop are able to access the live camera image via File / Import / Leica DFC camera.  A prerequisite for installing LAS was to install Leica DFC Twain 7.4.0

  We have followed the instructions for installing ImageJ including adding Twain_6.class to the plugins folder, adding Morina framework to ImageJ/jre/lib/ext folder.  ImageJ runs fine and we can carry out measurements on a saved image.  However we would like to be able to measure on a live image to improve efficiency (we are working with 3-D specimens that need to be correctly orientated for each measurement and it would be very tedious to have to capture and save an image with magnification info etc for each measurement).

  When we try to access the camera via plugins/TWAIN 6 we get an error mesage " SK.gnome.TWAIN.TwainException: No TWAIN Data Source installed "

  Can anyone please help - are we doing something wrong, or is it not possible to access a data source directly within ImageJ via Twain?  Does the code perhaps need to be tweaked to call the Leica DFC Twain 7.4.0 rather than Twain 6? Or do we need to install a Twain 6 driver and if so, where can we find this?  Please bear in mind that we are completely innocent of any programming know-how!

  Best wishes
  Peter





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