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thresholding selecting wrong areas

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thresholding selecting wrong areas

Uknalis, Joseph
I was trying Adjustable Watershed and then Biovoxxel Watershed irregular features  to separate large particles in an image. (w not much success)
Now when I threshold the background gets selected and not the objects

Clicked/unclicked dark background in THRESHOLD, no luck
Tried inverting, no luck
Cleared imagej.cfg, no luck
Upgraded version from 1.49q to 1.51i (via ij.jar replacement), no luck

Any ideas on how to restore this threshold function?

Thanks

Joe




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Re: thresholding selecting wrong areas

Straatman, Kees (Dr.)
Hi Joe,

Biovoxxel selects the setting Process > Binary > Options > Black background. You have to deselect this to restore the threshold function.

If you use Biovoxxel a lot you can add: run("Options...", "iterations=1 count=1"); to Edit > Options > Start Up to restores this setting when you restart ImageJ/Fiji

Best wishes

Kees

Dr Ir K.R. Straatman
Senior Experimental Officer
Advanced Imaging Facility
Centre for Core Biotechnology Services
University of Leicester
http://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/lite/aif



-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Uknalis, Joseph
Sent: 24 January 2017 16:20
To: [hidden email]
Subject: thresholding selecting wrong areas

I was trying Adjustable Watershed and then Biovoxxel Watershed irregular features  to separate large particles in an image. (w not much success) Now when I threshold the background gets selected and not the objects

Clicked/unclicked dark background in THRESHOLD, no luck Tried inverting, no luck Cleared imagej.cfg, no luck Upgraded version from 1.49q to 1.51i (via ij.jar replacement), no luck

Any ideas on how to restore this threshold function?

Thanks

Joe




This electronic message contains information generated by the USDA solely for the intended recipients. Any unauthorized interception of this message or the use or disclosure of the information it contains may violate the law and subject the violator to civil or criminal penalties. If you believe you have received this message in error, please notify the sender and delete the email immediately.

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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