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闪闪小妖
[image: image.png]
Dear
I am very sorry to interrupt, how to calculate the size distribution of the
holes in the figure, which plugin of image j to use? Thank you very much.

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Re: Holes in a fiber

Herbie
Dear poster,

obviously the holes are in a 3D-object (fiber) which means that the
holes suffer from geometric distortions in depth and their sizes can't
be accurately measured.

One way to overcome this problem is by geometrically flattening the
fiber but I doubt that this is possible with images of such low spatial
resolution.

Last but not least, the image appears to be copied from the literature.

Regards

Herbie

::::::::::::::::::::::::::::::::::::
Am 02.10.19 um 13:33 schrieb 闪闪小妖:
> [image: image.png]
> Dear
> I am very sorry to interrupt, how to calculate the size distribution of the
> holes in the figure, which plugin of image j to use? Thank you very much.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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Re: Holes in a fiber

Herbie
"It is also possible to calculate the size and distribution of the
apertures in the plane."

Please provide a corresponding image, otherwise it is impossible to
provide any useful help.

And please continue posting to the list!

Herbie

::::::::::::::::::::::::::::::::::::
Am 04.10.19 um 10:48 schrieb 闪闪小妖:

> Dear,
> Thank you for your reply. It is also possible to calculate the size and
> distribution of the apertures in the plane. How can I calculate it? Or
> which plugin to use
>
>
> Regards
>
> dong
>
> Herbie <[hidden email] <mailto:[hidden email]>> 于2019年10月4日周五 下
> 午5:13写道:
>
>     Dear poster,
>
>     obviously the holes are in a 3D-object (fiber) which means that the
>     holes suffer from geometric distortions in depth and their sizes can't
>     be accurately measured.
>
>     One way to overcome this problem is by geometrically flattening the
>     fiber but I doubt that this is possible with images of such low spatial
>     resolution.
>
>     Last but not least, the image appears to be copied from the literature.
>
>     Regards
>
>     Herbie
>
>     ::::::::::::::::::::::::::::::::::::
>     Am 02.10.19 um 13:33 schrieb 闪闪小妖:
>      > [image: image.png]
>      > Dear
>      > I am very sorry to interrupt, how to calculate the size
>     distribution of the
>      > holes in the figure, which plugin of image j to use? Thank you
>     very much.
>      >
>      > --
>      > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>      >
>
>     --
>     ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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Re: Holes in a fiber

CARL Philippe (LBP)
In reply to this post by Herbie
Dear Herbie,
What you are describing, i.e. of "geometrically flattening the fiber" this can very easily be done by the holographic microscope of Lyncee Tec:
https://www.lynceetec.com/
Such a system is for example used in industry within quality control of the measurement of the roughness of beads used in pens.
Indeed if the roughness of such beads is too big, you will put too much ink on your paper and for a too smooth bead there will be not enough ink deposited on the paper.
So they take a 3D picture of the bead (with a nm resolution in z) flatten the surface by means of the bead curvature (similarly to the world maps) and then measure the surface roughness.

My best regards,
Philippe

Philippe CARL
Laboratoire de Bioimagerie et Pathologies
UMR 7021 CNRS - Université de Strasbourg
Faculté de Pharmacie
74 route du Rhin
67401 ILLKIRCH
Tel : +33(0)3 68 85 41 84

----- Mail original -----
De: "Herbie" <[hidden email]>
À: "imagej" <[hidden email]>
Envoyé: Vendredi 4 Octobre 2019 10:12:45
Objet: Re: Holes in a fiber

Dear poster,

obviously the holes are in a 3D-object (fiber) which means that the
holes suffer from geometric distortions in depth and their sizes can't
be accurately measured.

One way to overcome this problem is by geometrically flattening the
fiber but I doubt that this is possible with images of such low spatial
resolution.

Last but not least, the image appears to be copied from the literature.

Regards

Herbie

::::::::::::::::::::::::::::::::::::
Am 02.10.19 um 13:33 schrieb 闪闪小妖:
> [image: image.png]
> Dear
> I am very sorry to interrupt, how to calculate the size distribution of the
> holes in the figure, which plugin of image j to use? Thank you very much.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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Re: Holes in a fiber

Herbie
Merci cher Philippe,

pour cette référence...

With respect to the sample image provided by the poster, I doubt that
its spatial resolution will aloow any meaningful flattening.

Bon weekend

Herbie

:::::::::::::::::::::::::::::::::::::::::::::::::
Am 04.10.19 um 11:32 schrieb CARL Philippe (LBP):

> Dear Herbie,
> What you are describing, i.e. of "geometrically flattening the fiber" this can very easily be done by the holographic microscope of Lyncee Tec:
> https://www.lynceetec.com/
> Such a system is for example used in industry within quality control of the measurement of the roughness of beads used in pens.
> Indeed if the roughness of such beads is too big, you will put too much ink on your paper and for a too smooth bead there will be not enough ink deposited on the paper.
> So they take a 3D picture of the bead (with a nm resolution in z) flatten the surface by means of the bead curvature (similarly to the world maps) and then measure the surface roughness.
>
> My best regards,
> Philippe
>
> Philippe CARL
> Laboratoire de Bioimagerie et Pathologies
> UMR 7021 CNRS - Université de Strasbourg
> Faculté de Pharmacie
> 74 route du Rhin
> 67401 ILLKIRCH
> Tel : +33(0)3 68 85 41 84
>
> ----- Mail original -----
> De: "Herbie" <[hidden email]>
> À: "imagej" <[hidden email]>
> Envoyé: Vendredi 4 Octobre 2019 10:12:45
> Objet: Re: Holes in a fiber
>
> Dear poster,
>
> obviously the holes are in a 3D-object (fiber) which means that the
> holes suffer from geometric distortions in depth and their sizes can't
> be accurately measured.
>
> One way to overcome this problem is by geometrically flattening the
> fiber but I doubt that this is possible with images of such low spatial
> resolution.
>
> Last but not least, the image appears to be copied from the literature.
>
> Regards
>
> Herbie
>
> ::::::::::::::::::::::::::::::::::::
> Am 02.10.19 um 13:33 schrieb 闪闪小妖:
>> [image: image.png]
>> Dear
>> I am very sorry to interrupt, how to calculate the size distribution of the
>> holes in the figure, which plugin of image j to use? Thank you very much.
>>
>> --
>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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Re: Holes in a fiber

Kenneth Sloan-3
To actually try to answer the OP's question...

I would first mask the cylinder(?) so that only the middle third is visible.  This may be close enough
to flat to give a reasonable approximation.

After that, we have dark(er) ovals on a light(er) background.  I don't do macros - but perhaps
someone can suggest a simple way to segment and measure dark ovals on a light background?

--
Kenneth Sloan
[hidden email]
Vision is the art of seeing what is invisible to others.

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Fwd:

闪闪小妖
In reply to this post by 闪闪小妖
---------- Forwarded message ---------
发件人: 闪闪小妖 <[hidden email]>
Date: 2019年10月2日周三 下午8:33
Subject:
To: <[hidden email]>


[image: image.png]
Dear
I am very sorry to interrupt, how to calculate the size distribution of the
holes in the figure, which plugin of image j to use? Thank you very much.

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

image.png (169K) Download Attachment