Re: ned help with new macro for backgournd subtraction from ROIs
If you hold the shift-key down when you select your ROI you can add
multiple ROIs. Does this work for you?
Hoiwever, if your background is uneven, you may want to try the core
background-subtraction function instead of subtracting a single value
across the image. (Process>Background subtraction)
> I know we have a great macro for background subtraction given a single ROI.
> But what i need is a way to subtract the background from the average intensity
> calculated from multiple ROIs.
> Basically what I need is a way to calculate the average intensity of multiple
> ROIs in a given image. (maybe 3 ROIs) Then use *that* average of the
> intensities to background subtract the entire image.
> IS that possible? if so, how? I could really use some help here. Any and all
> comments are welcome.
> thanks all in advance
Tony Collins, Ph.D.
Wright Cell Imaging Facility
Toronto Western Research Institute
13-407 McLaughlin Pavilion
399 Bathurst Street
Toronto, ON. M5T 2S8
tel. (416) 603 5367 fax: (416) 603 5745
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