# measuring intensities

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## measuring intensities

 I'm a new user of image j and I'm wondering if anyone knows if it's possible to measure the intensity of each individual pixel in an image that you want to anal yse? How can it be done? Thanks guys, Richard Murray Richard Murray BSc. Nanos cale Biophotonics Research Group, National Centre for Biomedical Engineering Sci ences, National University of Ireland, Galway, Phone: +353-91-524411 ext.5186 Em ail: [hidden email]
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## Re: measuring intensities

 > I'm a new user of image j and I'm wondering if anyone knows if it's > possible to > measure the intensity of each individual pixel in an image that you want > to anal > yse? How can it be done? >   The macro below might help:  h=getHeight();  w=getWidth();  for (a=0; a
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## Re: measuring intensities

 Not sure of the real question being asked, but placing the cursor over a pixel will read out the values in the ImageJ mssg field. If the intent was to list all the pixel intensities, it seems that "save as/ text image " option is the fastest route vs the 1-pixel region  of interests (ROI) approach below.          Selecting a ROI and using MEASURE is more common (built-in statistics vs spreadsheets). Listing values within ROI can be done with macros (see ImageJ macro links) At 05:55 8/9/2005, you wrote: > > I'm a new user of image j and I'm wondering if anyone knows if it's > > possible to > > measure the intensity of each individual pixel in an image that you want > > to anal > > yse? How can it be done? > > > >   The macro below might help: > > >  h=getHeight(); >  w=getWidth(); >  for (a=0; a      for (b=0; b           makeRectangle(a, b, 1, 1); >           run("Measure"); >      } >  } > > >    Regards, > >-- >Silviu Rusu, Ph.D. >Erasmus MC Rotterdam >Ee 1230 >Tel: +31 10 46 38094 >Fax: +31 10 40 89459 >Dr. Molewaterplein 50 >NL-3015 GE Rotterdam >email: [hidden email] >web: http://www2.eur.nl/fgg/neuro/Vytas Bindokas, Ph.D. Research Assoc. / Assoc. Prof., Director, BSD Light Microscopy Core Facility Dept Neurobiol Pharmacol Physiol MC0926 947 E 58th Street The University of Chicago Chicago IL 60637 Room J246A (SBRI) 773-834-9040 fax 773-702-5903 email [hidden email]   web site for LMCF: http://digital.bsd.uchicago.edu/index.html
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## Re: measuring intensities

 In reply to this post by Richard Murray-3 Hi, my intention is to measure the intenisty of each individual pixel without ha ving to select each individual pixel by hand. i.e as if each pixel were a sepera te element in a matrix, I want to know the value of each individual element in t he matrix. I hope this explains my problem more, Thanks, Richard ============= =Original message text=============== On Tue, 09 Aug 2005 12:32:18 +0100 vytas w rote: Not sure of the real question being asked, but placing the cursor over a pixel will read out the values in the ImageJ mssg field. If the intent was to list all the pixel intensities, it seems that "save as/ text image " option is the fastest route vs the 1-pixel region  of interests (ROI) approach below.           Selecting a ROI and using MEASURE is more common (built-in statistics vs spreadsheets). Listing values within ROI can be done with macros (see ImageJ ma cro links) At 05:55 8/9/2005, you wrote: > > I'm a new user of image j and I'm  wondering if anyone knows if it's > > possible to > > measure the intensity of each individual pixel in an image that you want > > to anal > > yse? How can it be done? > > > >   The macro below might help: > > >  h=getHeight(); >  w=getWid th(); >  for (a=0; a      for (b=0; b           makeRectan gle(a, b, 1, 1); >           run("Measure"); >      } >  } > > >    Regards, > > -- >Silviu Rusu, Ph.D. >Erasmus MC Rotterdam >Ee 1230 >Tel: +31 10 46 38094 >Fax : +31 10 40 89459 >Dr. Molewaterplein 50 >NL-3015 GE Rotterdam >email: s.rusu@er asmusmc.nl >web: http://www2.eur.nl/fgg/neuro/Vytas Bindokas, Ph.D. Research A ssoc. / Assoc. Prof., Director, BSD Light Microscopy Core Facility Dept Neurobio l Pharmacol Physiol MC0926 947 E 58th Street The University of Chicago Chicago I L 60637 Room J246A (SBRI) 773-834-9040 fax 773-702-5903 email [hidden email] chicago.edu   web site for LMCF: http://digital.bsd.uchicago.edu/index.html  === ========End of original message text=========== Richard Murray BSc. Nanoscale  Biophotonics Research Group, National Centre for Biomedical Engineering Science s, National University of Ireland, Galway, Phone: +353-91-524411 ext.5186 Email:  [hidden email]