how to retain a line selection

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how to retain a line selection

Alpesh Khushalchand Shukla
I have images which have line like features, and I need to measure length of each line segment. I am
doing this manually, i.e., measuring the length of each line segment, looking up the length on the
toolbar, and typing it in Kaleidagraph. I cannot use the automatic measure because my TEM images
are not very high-contrast images, and thresholding would give a very high amount of error.

My problem: Is there a way to retain a line selection? When I go to the next feature, the last one
disappears. this creates confusion as I have to keep a close track of which line segments I have
measured. If they can be marked, the job (which is really tedious) will become a little easier.

Thanks

Alpesh
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Re: how to retain a line selection

Jeff Brandenburg
The ROI Manager plugin (Analyze: Tools: ROI Manager...) lets you
maintain a list of selections, but as distributed it doesn't let you
display all selections at once.

I did a modified version of ROI Manager several years ago that allowed
me to display and measure several different ROIs at once, but it
involved a lot of clumsy and heavy-handed modifications to other
classes (including, among others, ImagePlus), and so it wasn't really
suitable for release.

Would it work in your application to mark (Edit: Draw) each selection
after you measure it?  It would modify your working copy of the image,
but at least you'd be able to see what you've done.

On Aug 15, 2005, at 2:50 AM, Alpesh Khushalchand Shukla wrote:

> I have images which have line like features, and I need to measure
> length of each line segment. I am
> doing this manually, i.e., measuring the length of each line segment,
> looking up the length on the
> toolbar, and typing it in Kaleidagraph. I cannot use the automatic
> measure because my TEM images
> are not very high-contrast images, and thresholding would give a very
> high amount of error.
>
> My problem: Is there a way to retain a line selection? When I go to
> the next feature, the last one
> disappears. this creates confusion as I have to keep a close track of
> which line segments I have
> measured. If they can be marked, the job (which is really tedious)
> will become a little easier.
>
> Thanks
>
> Alpesh
>
--
        -jeffB (Jeff Brandenburg, Duke Center for In-Vivo Microscopy)
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Re: how to retain a line selection

Robert Baer
In reply to this post by Alpesh Khushalchand Shukla
You may want to download Wayne's "Measure and Label" plugin:
http://rsb.info.nih.gov/ij/plugins/measure-label.html

It is most convenient to use if you assign a shortcut key to the plugin.
Rob

----- Original Message -----
From: "Alpesh Khushalchand Shukla" <[hidden email]>
To: <[hidden email]>
Sent: Monday, August 15, 2005 1:50 AM
Subject: how to retain a line selection


> I have images which have line like features, and I need to measure length
of each line segment. I am
> doing this manually, i.e., measuring the length of each line segment,
looking up the length on the
> toolbar, and typing it in Kaleidagraph. I cannot use the automatic measure
because my TEM images
> are not very high-contrast images, and thresholding would give a very high
amount of error.
>
> My problem: Is there a way to retain a line selection? When I go to the
next feature, the last one
> disappears. this creates confusion as I have to keep a close track of
which line segments I have
> measured. If they can be marked, the job (which is really tedious) will
become a little easier.
>
> Thanks
>
> Alpesh
>
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Re: how to retain a line selection

Alpesh Khushalchand Shukla
I found this Measure and label macro useful. One more
question: how can I assign a shortcut key? (so that I
don't have to go to plugins-macro-measure&label)
everytime?

Alpesh


--- Robert Baer <[hidden email]> wrote:

> You may want to download Wayne's "Measure and Label"
> plugin:
>
http://rsb.info.nih.gov/ij/plugins/measure-label.html

>
> It is most convenient to use if you assign a
> shortcut key to the plugin.
> Rob
>
> ----- Original Message -----
> From: "Alpesh Khushalchand Shukla"
> <[hidden email]>
> To: <[hidden email]>
> Sent: Monday, August 15, 2005 1:50 AM
> Subject: how to retain a line selection
>
>
> > I have images which have line like features, and I
> need to measure length
> of each line segment. I am
> > doing this manually, i.e., measuring the length of
> each line segment,
> looking up the length on the
> > toolbar, and typing it in Kaleidagraph. I cannot
> use the automatic measure
> because my TEM images
> > are not very high-contrast images, and
> thresholding would give a very high
> amount of error.
> >
> > My problem: Is there a way to retain a line
> selection? When I go to the
> next feature, the last one
> > disappears. this creates confusion as I have to
> keep a close track of
> which line segments I have
> > measured. If they can be marked, the job (which is
> really tedious) will
> become a little easier.
> >
> > Thanks
> >
> > Alpesh
> >
>


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Re: how to retain a line selection

Wayne Rasband
> I found this Measure and label macro useful. One
>  more  question: how can I assign a shortcut key?
> (so that I  don't have to go to plugins-macro-measure&label)
> everytime?

Change the first line of the macro from

       macro "Measure and label" {

to

       macro "Measure and label [F1]" {

reinstall it and you will be able to run it by pressing the F1 key.

More information and examples can be found at

    http://rsb.info.nih.gov/ij/developer/macro/macros.html#shortcuts

-wayne
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