回复:how to generate random spots in 3D images by macro

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回复:how to generate random spots in 3D images by macro

Yan xiaolong
Hi wanzhu:did you means you want to generate balls in random position? you can generate white pixels in every slice, then do a distance transform 3D (need another plugins you can find in the official site), then do a threshold. you will got some 3d ball in the image stack.
yan xiaolong
----- 原始邮件 -----
发件人:Wanzhu <[hidden email]>
收件人:[hidden email]
主题:how to generate random spots in 3D images by macro
日期:2017年08月03日 00点46分


I have a 3D image and I demarcated a 3D ROI. I want to generate 200 spots
(each spot's diameter is 4 um which represent cells) only placed in the 3D
ROI. So I can further import this images to Imaris and calculate the
distance between these simulated spots and actual blood vessels in ROI.  
I have no experience in Macro,  I really appreciate it if you can help me.
Thank you.
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