I have done some fluorescence experiments as a function of the time, and I need merge the images (Image left) and create a map with the fluorescence intensity (image right). Anyone knows if it is possible to do this using Imaje J? Or other software that I can do it.
Hi Thiago, It seems to me that what you need is to convert the image to
8-bit, and then provide a look-up-table that will change color in
proportion to the intensity. Basically, you should:
1. convert the image to 8-bit. (Image>type>8-bit), adjust contrast and
brightness if necessary.
2. apply a look-up-table from the selection that comes from ImageJ or
create your own,
a, go to Image>color>display LUTS
b. note the LUT that you think will be best for your data. (I used the
c. go to File>import>lut, and select that lut (It will be in the ImageJ
3. Convert the image back to RGB color (Image>type>RGB color)
4. Resave it
You should get something like the one I have attached.
This will not be a quantitative display, since the brightness has been
adjusted, but it is possible to set up some standards so that specific
colors correspond to specific intensities --Be aware of all of the caveats,
generously scattered through this list, about quantitating fluorescence.