calibration problem- new in IJ

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calibration problem- new in IJ

Esra Sengul (Student)
Dear All,

I'm new in IJ analysis and tried to measure the area of cells. I set the
unit of length as pixel a distance in pixels to 0, known to distance to
0.00, pixel aspect ratio to 1 and global is chosen. The images are 866x718
pixels, RGB.

Now my question is: is it possible to convert my results into µm²?

I'd appreciate any comments!
Best regards,
Esra

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Re: calibration problem- new in IJ

Cammer, Michael-2
There are a few tutorials online regarding this.

Here are two of them.
 https://imagej.net/Spatial_Calibration
http://microscopy.berkeley.edu/courses/dib/sections/04IPIII/IJsetscale.html

Found using this search
https://www.google.com/search?q=set+spatial+scale+imagej& 

Cheers-

Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY  10016
Office: 646-501-0567 Cell: 914-309-3270  [hidden email]  
http://nyulmc.org/micros  http://microscopynotes.com/
 





-----Original Message-----
From: Esra Sengul (Student) <[hidden email]>
Sent: Tuesday, February 4, 2020 2:56 PM
To: [hidden email]
Subject: calibration problem- new in IJ

[EXTERNAL]

Dear All,

I'm new in IJ analysis and tried to measure the area of cells. I set the unit of length as pixel a distance in pixels to 0, known to distance to 0.00, pixel aspect ratio to 1 and global is chosen. The images are 866x718 pixels, RGB.

Now my question is: is it possible to convert my results into µm²?

I'd appreciate any comments!
Best regards,
Esra

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