Variable ROI in Time Series

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Variable ROI in Time Series

Javier Casas
Dear community of ImageJ users,

I would like to measure translocation of a plasma membrane labeling to the
cytosol over the time, to that end its easy to make a binary mask of the
membrane, and to register the images to the first frame with the turbo reg
plugin, but shape of the membrane/cells still have some movement between
frames, how could I select an adaptive ROI for the time series based on the
Binary mask?

I hope you guys can help me, attached you'll find the image and the mask
Thanks a lot in advanced

Javier

<http://imagej.1557.x6.nabble.com/file/t381515/DUP_PKCa_LPS_Registered_Green.png>
<http://imagej.1557.x6.nabble.com/file/t381515/DUP_PKCa_LPS_Registered_Green-1.png>




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