Overlaying images with different Scales

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Overlaying images with different Scales

bjohnson
Hello All, I would like to over lay two SEM images over one another, which have different images sizes and scales. Both images at the same magnification, but using different machines, and have different Pixel Scales, and total pixel counts. Image one = 15.17 pixels per um (Image Size = 1236x862 pixels) Image two = 4.90 pixels per um (Image Size = 554x412 pixels) I would like to over lay one image over the other. Is there a way I can convert the images to the same scale, so that I can do an overlay? Any help would be greatly appreciated.
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Re: Overlaying images with different Scales

Jeremy Adler-2
-----Original Message-----
From: Jeremy Adler
Sent: den 27 juni 2016 09:31
To: '[hidden email]'
Subject: RE: Overlaying images with different Scales

Image\Scale allows you to expand or shrink an image, so can be used to create images with same sized pixels.
The image with 15um pixels needs to be rescaled by around 3x

The two images do not have the same resolution but can be aligned and overlayed.





-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of bjohnson
Sent: den 26 juni 2016 02:09
To: [hidden email]
Subject: Overlaying images with different Scales

Hello All,I would like to over lay two SEM images over one another, which have different images sizes and scales.Both images at the same magnification, but using different machines, and have different Pixel Scales, and total pixel counts.Image one = 15.17 pixels per um (Image Size =
1236x862 pixels)Image two = 4.90 pixels per um (Image Size = 554x412 pixels)I would like to over lay one image over the other. Is there a way I can convert the images to the same scale, so that I can do an overlay?Any help would be greatly appreciated.



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Re: Overlaying images with different Scales

Derek Schaller
In reply to this post by bjohnson
The fully functional testing version of GIMP runs with the MECL backend in 32 bit floating point and can handle just about any image file up to 32 bit now. The stable version runs in 8 bit color depth.
I would use GIMP for anything you have trouble with doing in imageJ regarding specific tasks such as scaling.
If you search gimp 2.9.3 you will find various testing versions uploaded at partha.com, one of the gimp developers. If you are running linux, you can get ppa's for version 2.9.2 with constant improvements coming in. The open source community is really taking off.
Derek
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Re: Overlaying images with different Scales

Christian Goosmann-2
In reply to this post by bjohnson
Hello
I would use TrakEM2. It comes with FIJI distribution of ImageJ. I would
import both images to different planes of a 2-plane project and use
manual aligning with landmarks. Afterwards you can export to a 2-slices
stack of the aligned images with adjusted canvas as needed. It is a bit
of a learnig curve, but good documentation exists.
hth
Christian




--- Christian Goosmann Mikroskopie Max-Planck-Institut für
Infektionsbiologie Campus Charité Mitte Charitéplatz 1 10117 Berlin
Tel.: +49 30 28460 388

bjohnson wrote:

> Hello All,I would like to over lay two SEM images over one another, which
> have different images sizes and scales.Both images at the same
> magnification, but using different machines, and have different Pixel
> Scales, and total pixel counts.Image one = 15.17 pixels per um (Image Size =
> 1236x862 pixels)Image two = 4.90 pixels per um (Image Size = 554x412
> pixels)I would like to over lay one image over the other. Is there a way I
> can convert the images to the same scale, so that I can do an overlay?Any
> help would be greatly appreciated.
>
>
>
> --
> View this message in context: http://imagej.1557.x6.nabble.com/Overlaying-images-with-different-Scales-tp5016728.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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