Alex here, I am PhD graduate student with a quick image normalization question. I have been doing some imaging of the organ of asymmetry in zebrafish (Kupffer's Vesicle) using GCaMP to detect calcium elevations around this organ and using mCherry expressed 1:1 in order to control for cell to cell expression variation.
My question is what is the best method to "normalize" my GCaMP signal using mCherry signal. I initially though something like the Image Process: Divide function would give me what I need but now reading around the net I am not so sure. Any and all advice is welcome and appreciated thanks!!