Multiple-channel stack registration

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Multiple-channel stack registration

Sho Fujisawa
I have serial sections that need to be aligned. Usually I use Stack Registration with SIFT, but these sections are fluorescently stained with total of 5 different channels. This and other plugins have issues with images that are not 8-bit or 24-bit. Once a 5-channel image is converted to 24-bit, I can't convert them back to 5 channels. There are over 20 sections to align.

Any advice, anyone?

Sho Fujisawa
Senior Digital Microscopist
Molecular Cytology Core Facility
Memorial Sloan-Kettering Cancer Center
417 E68th St. ZRC-1840
New York, NY 10065


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