Image Analysis using Image J

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Image Analysis using Image J

Sharat Pankajavihar Sasi
Hello,
       I am a new user of the Image J software and had few queries on the
application I am looking for. My issue is as stated below:

"I have Calibration pictures that were taken at 40X lense and with different
zooms on the camera say 50, 77, 88 etc. These images taken need to be
calibrated from pixels to microns in the Image J software if possible. Then,
the stained images of the tissue taken with the same lens and zoom under the
same miscroscope need to be added in the software and the thickness and
diameter need to be measured in microns."

Can this be done with Scion imaging software? Could you please advice me on the
same? If its not possible can anyone please suggest me an alternative?

Thanking You,

Sharath. P. Sasi

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Re: Image Analysis using Image J

Mark McNabb
On Thu, 18 Aug 2005, Mark David McNabb wrote:

Hi Sharat,

I have done this in both ImageJ, NIH Image, and Scion Image.  It is
relatively straight forward if you do it manually.  You would just use the
calibration images - drawing straight segmented lines across your known
calibration grid or sphere distance - and the "Analyze -> Set Scale" menu to
calculate the calibration numbers for each zoom setting.  Then when you open
an image at a certain zoom and magnification, you would just go to that same
menu item and enter the calibration number for those settings. Then you would
just draw straight segments across your tissue structure and use the "Analyze
-> Measure" to get your measurement.


Mark.

> On Thu, 18 Aug 2005, Sharat Pankajavihar Sasi wrote:
>
> Hello,
>       I am a new user of the Image J software and had few queries on the
> application I am looking for. My issue is as stated below:
>
> "I have Calibration pictures that were taken at 40X lense and with
> different
> zooms on the camera say 50, 77, 88 etc. These images taken need to be
> calibrated from pixels to microns in the Image J software if possible.
> Then,
> the stained images of the tissue taken with the same lens and zoom under
> the
> same miscroscope need to be added in the software and the thickness and
> diameter need to be measured in microns."
>
> Can this be done with Scion imaging software? Could you please advice me on
> the
> same? If its not possible can anyone please suggest me an alternative?
>
> Thanking You,
>
> Sharath. P. Sasi
>
> ----------------------------------------------------------------
> This message was sent using IMP, the Internet Messaging Program.
>
>
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Re: Image Analysis using Image J

Sharat Pankajavihar Sasi
Hi Mark,
           Thanks a lot for your reply. I will do as you say and try out using
Scion Image. If I have any more queries I will get back with you.

Thanks a lot Once again!!!

Sharath

Quoting Mark David McNabb <[hidden email]>:

> On Thu, 18 Aug 2005, Mark David McNabb wrote:
>
> Hi Sharat,
>
> I have done this in both ImageJ, NIH Image, and Scion Image.  It is
> relatively straight forward if you do it manually.  You would just
> use the calibration images - drawing straight segmented lines across
> your known calibration grid or sphere distance - and the "Analyze ->
> Set Scale" menu to calculate the calibration numbers for each zoom
> setting.  Then when you open an image at a certain zoom and
> magnification, you would just go to that same menu item and enter the
> calibration number for those settings. Then you would just draw
> straight segments across your tissue structure and use the "Analyze
> -> Measure" to get your measurement.
>
>
> Mark.
>
>> On Thu, 18 Aug 2005, Sharat Pankajavihar Sasi wrote:
>>
>> Hello,
>>       I am a new user of the Image J software and had few queries on the
>> application I am looking for. My issue is as stated below:
>>
>> "I have Calibration pictures that were taken at 40X lense and with different
>> zooms on the camera say 50, 77, 88 etc. These images taken need to be
>> calibrated from pixels to microns in the Image J software if possible. Then,
>> the stained images of the tissue taken with the same lens and zoom under the
>> same miscroscope need to be added in the software and the thickness and
>> diameter need to be measured in microns."
>>
>> Can this be done with Scion imaging software? Could you please
>> advice me on the
>> same? If its not possible can anyone please suggest me an alternative?
>>
>> Thanking You,
>>
>> Sharath. P. Sasi
>>
>> ----------------------------------------------------------------
>> This message was sent using IMP, the Internet Messaging Program.
>>
>>
>



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