Deconvolution

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Deconvolution

Fairy
Hiii all .

I had an 8bit image which I converted  to 16 bit.. The intensity  range
initially  was 0-255...after converting  it to 16 bit, it's now
0-65535...after doing Richardson Lucy deconvolution in deconvolutionlab,
 the signal intensity is reduced to 7000 from 65535....the background
 intensity  is increasing.... Thus overall s/N is increasing .... But I am
not understanding  why  the signal intensity  is dropping  like this!!
Please help!!!

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Re: Deconvolution

Justin Price
Not really sure what the end answer is but your first mistake was up-converting from 8 to 16 bit - was there a particular reason for this?

> On Sep 10, 2017, at 1:49 PM, Ash Fairy <[hidden email]> wrote:
>
> now

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Re: Deconvolution

Fairy
Yaa... Well... I was having  just that 8bit image with me and I had to do
with it!!



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