De-stack and re-stack

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De-stack and re-stack

Gabriela Venturini
Hi,

I am having problems with TIFF images output from an acquisition
timecourse. The software output each timepoint acquisition as a stack of
two images. I need to unstack these two images to restack with the entire
time course. any easy ways to do this? I cant manually unstack then
re-stack as it is a timecourse of 200 different acquisitions.

Tks!

All best
--
Gabriela Venturini, MSc, PhD Student.
Lab Genetics and Molecular Cardiology/LIM 13
Heart Institute (InCor)/Univ of Sao Paulo Med Sch
44 Dr. Eneas C Aguiar Ave
05403-000
Sao Paulo SP
Brazil
Phone: 55-11-2661-5993
Skype: venturini.gabriela
E-mail: [hidden email] <[hidden email]>
           [hidden email]

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Re: De-stack and re-stack

Cammer, Michael
Can't you concatenate all stacks and then resample as needed by either reducing with and without the first slice deleted or by turning into a hyperstack and splitting?

=*===========================================================*=
 Michael Cammer, DART Microscopy Laboratory, NYU Langone Medical Center
    Cell:  914-309-3270 (this is for calling, not texting)    Office: Skirball 2nd Floor main office, back right
      http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/


-----Original Message-----
From: Gabriela Venturini [mailto:[hidden email]]
Sent: Wednesday, March 22, 2017 6:25 PM
To: [hidden email]
Subject: De-stack and re-stack

Hi,

I am having problems with TIFF images output from an acquisition timecourse. The software output each timepoint acquisition as a stack of two images. I need to unstack these two images to restack with the entire time course. any easy ways to do this? I cant manually unstack then re-stack as it is a timecourse of 200 different acquisitions.

Tks!

All best
--
Gabriela Venturini, MSc, PhD Student.
Lab Genetics and Molecular Cardiology/LIM 13 Heart Institute (InCor)/Univ of Sao Paulo Med Sch
44 Dr. Eneas C Aguiar Ave
05403-000
Sao Paulo SP
Brazil
Phone: 55-11-2661-5993
Skype: venturini.gabriela
E-mail: [hidden email] <[hidden email]>
           [hidden email]

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