Creating boundary on merged cells

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Creating boundary on merged cells

Rohitesh Gupta
Hi All,

Thank you for involving me in the imagej forum.
I have a query, how could one create boundary/segmentation based on cell membrane ? I am attaching here an image on which I would like to create boundary.
Any help will be much appreciated.

Thanks,
Rohitesh

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Re: Creating boundary on merged cells

Cammer, Michael-2
It looks like you stained for f-actin in green which does not mark the cell boundaries.  I would label cell membranes or proteins localized specifically to cell membranes.
Alternatively, depending what you need to measure and how many measurements you need to make, it may be far more economical to manually trace.
Cheers-

Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY  10016
C: 914-309-3270  [hidden email]  http://nyulmc.org/micros  http://microscopynotes.com/ 




-----Original Message-----
From: ImageJ Interest Group <[hidden email]> On Behalf Of Rohitesh Gupta
Sent: Monday, April 8, 2019 12:17 AM
To: [hidden email]
Subject: Creating boundary on merged cells

Hi All,

Thank you for involving me in the imagej forum.
I have a query, how could one create boundary/segmentation based on cell membrane ? I am attaching here an image on which I would like to create boundary.
Any help will be much appreciated.

Thanks,
Rohitesh

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Re: Creating boundary on merged cells

Yaman
In reply to this post by Rohitesh Gupta
You can try color threshold tool.. the green color can be segmented in Lab
color space by setting the suitable limits of a* channel..
Regards



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