Correcting Intensity Measurements for Cell Rounding

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Correcting Intensity Measurements for Cell Rounding

Chris O'Connell
Hi All,

We want to plot integrated fluorescent intensity over time in cultured
cells.  The cells are rounding during the recording.  Is there a generally
accepted way to correct for this using the area of the cells?

Thanks in advance.
Chris O'Connell



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Re: Correcting Intensity Measurements for Cell Rounding

Cammer, Michael
You could try the following.
Microscopy  with a low NA lens to capture entire depth of cell in one image.  
Measure area & mean intensity of each cell, area * intensity.  

Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY  10016
[hidden email]  http://nyulmc.org/micros  http://microscopynotes.com/ 
Voice direct only, no text or messages:  1-914-309-3270 and 1-646-501-0567



-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Chris O'Connell
Sent: Friday, August 10, 2018 9:30 AM
To: [hidden email]
Subject: Correcting Intensity Measurements for Cell Rounding

Hi All,

We want to plot integrated fluorescent intensity over time in cultured
cells.  The cells are rounding during the recording.  Is there a generally
accepted way to correct for this using the area of the cells?

Thanks in advance.
Chris O'Connell



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