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I'm trying to threshold 16 bit images but the outcome of the operation looks like the threshold values were both set to the max value so i guess that the 16 bit value i enter is interpretet as 8 bit and simply set to 255 resulting in a completely empty image. (i could reproduce the error by setting the threshold to max manually.
run("Convert to Mask");
were Th1 and Th2 are the threshold values. Operation works fine with 8-bit .jpg
i solved it by converting the 16 bt into 8bit image but i think that should not be necessary.