Calculating B-Gal Staining Cells

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Calculating B-Gal Staining Cells

Anton R
Hi All,

I would like to ask how to calculate B-Galactosidase stained cells by using imageJ. As I am new with this software, I face difficulties when using it. Moreover, the characteristic of green-blue color cell is not homogenous in one picture. Hence, I need your help:
i.  Are these steps correct:
substract threshold-->run color deconvolution--> determine the ROI.
However, the cell color based on ROI is different with the original photo. If there are 3  pictures provided by ROI vector matrix, which one should I use to calculate the staining cells?
ii. How to calculate the staining cells, can it be analyzed directly from one of the ROI Vector matrix with "analyze particle"?

Thank you.

Regard,

Anton

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