I would like to ask how to calculate B-Galactosidase stained cells by using imageJ. As I am new with this software, I face difficulties when using it. Moreover, the characteristic of green-blue color cell is not homogenous in one picture. Hence, I need your help:
i. Are these steps correct:
substract threshold-->run color deconvolution--> determine the ROI.
However, the cell color based on ROI is different with the original photo. If there are 3 pictures provided by ROI vector matrix, which one should I use to calculate the staining cells?
ii. How to calculate the staining cells, can it be analyzed directly from one of the ROI Vector matrix with "analyze particle"?