Background substracting except for a ROI

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Background substracting except for a ROI

Adrián Villalba
Dear all,

I use to substract background in fluorescence images (1 channel) by
substracting the mean value setted by measurements. In the case that the
background is not uniform i would like to know if it possible to substract
the entire image without the ROI from the original image.

 Thank you all in advance,

--

   - Adrián Villalba Felipe.
   https://es.linkedin.com/in/adrianvillalba

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Re: Background substracting except for a ROI

Straatman, Kees (Dr.)
Dear Adrian,

You can use Process > Image Calculator to subtract images. There is also the option to use Process > Subtract Background... what uses a rolling background approach to correct uneven illuminated background, see: https://imagej.nih.gov/ij/docs/guide/146-29.html#toc-Subsection-29.14

Kees

Dr Ir K.R. Straatman
Senior Experimental Officer
Advanced Imaging Facility
Centre for Core Biotechnology Services
University of Leicester
http://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/lite/aif


-----Original Message-----
From: Adrián Villalba [mailto:[hidden email]]
Sent: 07 June 2017 09:35
To: [hidden email]
Subject: Background substracting except for a ROI

Dear all,

I use to substract background in fluorescence images (1 channel) by substracting the mean value setted by measurements. In the case that the background is not uniform i would like to know if it possible to substract the entire image without the ROI from the original image.

 Thank you all in advance,

--

   - Adrián Villalba Felipe.
   https://es.linkedin.com/in/adrianvillalba

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html
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