Antibody Array Analysis/ multispot analysis

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Antibody Array Analysis/ multispot analysis

Elbai Samira Mohamed
Dear User List,

I am new to ImageJ and I have a question hopefully someone can help me with:
I am analysing an antibody array with multiple spots and I want to use the same mask size/area for each spot is there a way of saving a template mask and moving it across the array to analyse each individual spot with the same mask?

Many thanks for your feedback, Samira
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Re: Antibody Array Analysis/ multispot analysis

Straatman, Kees (Dr.)
Dear Samira,

Maybe the dot blot analyser (http://image.bio.methods.free.fr/dotblot.html) can do the job?

Kees

Dr Ir K.R. Straatman
Senior Experimental Officer
Advanced Imaging Facility
Centre for Core Biotechnology Services
University of Leicester
http://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/lite/aif


-----Original Message-----
From: Elbai Samira Mohamed [mailto:[hidden email]]
Sent: 14 June 2017 12:26
To: [hidden email]
Subject: Antibody Array Analysis/ multispot analysis

Dear User List,

I am new to ImageJ and I have a question hopefully someone can help me with:
I am analysing an antibody array with multiple spots and I want to use the same mask size/area for each spot is there a way of saving a template mask and moving it across the array to analyse each individual spot with the same mask?

Many thanks for your feedback, Samira
--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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Re: Antibody Array Analysis/ multispot analysis

JOEL B. SHEFFIELD
I would be interested in this routine as well.  I wonder, does anyone know
if this plugin implements a local background subtraction algorithm?



Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839 <(215)%20204-8839>
e-mail: [hidden email]
URL:  *https://bio.cst.temple.edu/~jbs/ <https://bio.cst.temple.edu/~jbs/>
<http://tinyurl.com/khbouft>*

On Wed, Jun 14, 2017 at 7:40 AM, Straatman, Kees (Dr.) <[hidden email]
> wrote:

> Dear Samira,
>
> Maybe the dot blot analyser (http://image.bio.methods.free.fr/dotblot.html)
> can do the job?
>
> Kees
>
> Dr Ir K.R. Straatman
> Senior Experimental Officer
> Advanced Imaging Facility
> Centre for Core Biotechnology Services
> University of Leicester
> http://www2.le.ac.uk/colleges/medbiopsych/facilities-and-ser
> vices/cbs/lite/aif
>
>
> -----Original Message-----
> From: Elbai Samira Mohamed [mailto:[hidden email]]
> Sent: 14 June 2017 12:26
> To: [hidden email]
> Subject: Antibody Array Analysis/ multispot analysis
>
> Dear User List,
>
> I am new to ImageJ and I have a question hopefully someone can help me
> with:
> I am analysing an antibody array with multiple spots and I want to use the
> same mask size/area for each spot is there a way of saving a template mask
> and moving it across the array to analyse each individual spot with the
> same mask?
>
> Many thanks for your feedback, Samira
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html
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