3 color linescan in 12 or 16 bit?

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3 color linescan in 12 or 16 bit?

Monique Vasseur
Hi to all,

Is there a plugin that can show on the same linescan graph data of different colors of 12 or 16 bit images (without being changed in 8 bit RGB)?
Many thanks in advance.

Cordially,

Monique

Monique Vasseur
Responsable de la plateforme de microscopie photonique
Département de biochimie et médecine moléculaire
Pavillon Roger-Gaudry, local D-333
Université de Montréal
2900, boul. Édouard Montpetit
Montréal, QC    H3T 1J4   Canada
Tél. (514) 343-6111 poste 5148

https://biochimie.umontreal.ca/plateformes-scientifiques-bmm/microscopie/


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Re: 3 color linescan in 12 or 16 bit?

Volker Baecker
Hi Monique,

you can add data from one plot, created by plot-profile, to another
plot. On the window of the plot, click the "Data >>" button and select
"add from plot".

It is also easy to do in the macro language, see the "Plot." commands.

It works with 16 bit images.

See at the bottom of

/MontpellierRessourcesImagerie/imagej_macros_and_scripts/wiki/MRI_Heights_of_Surfaces_Tools

for an example.

Best regards,
Volker Baecker

Vasseur Monique:

> Hi to all,
>
> Is there a plugin that can show on the same linescan graph data of different colors of 12 or 16 bit images (without being changed in 8 bit RGB)?
> Many thanks in advance.
>
> Cordially,
>
> Monique
>
> Monique Vasseur
> Responsable de la plateforme de microscopie photonique
> Département de biochimie et médecine moléculaire
> Pavillon Roger-Gaudry, local D-333
> Université de Montréal
> 2900, boul. Édouard Montpetit
> Montréal, QC    H3T 1J4   Canada
> Tél. (514) 343-6111 poste 5148
>
> https://biochimie.umontreal.ca/plateformes-scientifiques-bmm/microscopie/
>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
Volker Bäcker
Bioimage Analyst
Montpellier Ressources Imagerie
[hidden email]

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Re: 3 color linescan in 12 or 16 bit?

Herbie
In reply to this post by Monique Vasseur
Good day Monique,

you don't tell us the format of your images. Are these color stacks?

If yes, then it is is possible.
12bit images generally come as 16bit images.
ImageJ accepts 8bit, 16bit, rgb24bit, 32bit and to a certain extent
rgb48bit images.

It would be helpful, if you can make a sample image accessible via a
dropbox. Perhpas as ZIP-archive.

Regards

Herbie

:::::::::::::::::::::::::::::::::::::::::::::
Am 15.11.18 um 15:20 schrieb Vasseur Monique:

> Hi to all,
>
> Is there a plugin that can show on the same linescan graph data of different colors of 12 or 16 bit images (without being changed in 8 bit RGB)?
> Many thanks in advance.
>
> Cordially,
>
> Monique
>
> Monique Vasseur
> Responsable de la plateforme de microscopie photonique
> Département de biochimie et médecine moléculaire
> Pavillon Roger-Gaudry, local D-333
> Université de Montréal
> 2900, boul. Édouard Montpetit
> Montréal, QC    H3T 1J4   Canada
> Tél. (514) 343-6111 poste 5148
>
> https://biochimie.umontreal.ca/plateformes-scientifiques-bmm/microscopie/
>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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Re: 3 color linescan in 12 or 16 bit?

Krs5
In reply to this post by Monique Vasseur
Dear Monique,

The https://imagej.nih.gov/ij/macros/Plot_Multicolor.txt macro might be of use for this.

Best wishes

Kees


Dr Ir K.R. Straatman
Senior Experimental Officer
Advanced Imaging Facility
Centre for Core Biotechnology Services
University of Leicester
www.le.ac.uk/advanced-imaging-facility


-----Original Message-----
From: Vasseur Monique <[hidden email]>
Sent: 15 November 2018 14:20
To: [hidden email]
Subject: 3 color linescan in 12 or 16 bit?

Hi to all,

Is there a plugin that can show on the same linescan graph data of different colors of 12 or 16 bit images (without being changed in 8 bit RGB)?
Many thanks in advance.

Cordially,

Monique

Monique Vasseur
Responsable de la plateforme de microscopie photonique Département de biochimie et médecine moléculaire Pavillon Roger-Gaudry, local D-333 Université de Montréal 2900, boul. Édouard Montpetit
Montréal, QC    H3T 1J4   Canada
Tél. (514) 343-6111 poste 5148

https://biochimie.umontreal.ca/plateformes-scientifiques-bmm/microscopie/


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Re: 3 color linescan in 12 or 16 bit?

jmutterer
Hi Monique,
This macro tool might also work for creating profile plots from
multichannel images with higher bitdepth.
https://gist.github.com/mutterer/4a8e226fbe55e8e682a1

Jerome.

Le jeu. 15 nov. 2018 à 17:54, Straatman, Kees (Dr.) <[hidden email]>
a écrit :

> Dear Monique,
>
> The https://imagej.nih.gov/ij/macros/Plot_Multicolor.txt macro might be
> of use for this.
>
> Best wishes
>
> Kees
>
>
> Dr Ir K.R. Straatman
> Senior Experimental Officer
> Advanced Imaging Facility
> Centre for Core Biotechnology Services
> University of Leicester
> www.le.ac.uk/advanced-imaging-facility
>
>
> -----Original Message-----
> From: Vasseur Monique <[hidden email]>
> Sent: 15 November 2018 14:20
> To: [hidden email]
> Subject: 3 color linescan in 12 or 16 bit?
>
> Hi to all,
>
> Is there a plugin that can show on the same linescan graph data of
> different colors of 12 or 16 bit images (without being changed in 8 bit
> RGB)?
> Many thanks in advance.
>
> Cordially,
>
> Monique
>
> Monique Vasseur
> Responsable de la plateforme de microscopie photonique Département de
> biochimie et médecine moléculaire Pavillon Roger-Gaudry, local D-333
> Université de Montréal 2900, boul. Édouard Montpetit
> Montréal, QC    H3T 1J4   Canada
> Tél. (514) 343-6111 poste 5148
>
> https://biochimie.umontreal.ca/plateformes-scientifiques-bmm/microscopie/
>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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